Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

BNIP3L

Uniprot No.

O60238

Research Area

Cancer

Alternative Names

Adenovirus E1B19k binding protein B5; Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kd protein interacting protein 3a; BCL2/adenovirus E1B 19 kDa protein interacting protein 3A; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; BCL2/adenovirus E1B 19kDa interacting protein 3 like; BNI3L_HUMAN; BNIP3a; BNIP3H; BNIP3L; BNIP3L protein; NIP3 like protein X; NIP3-like protein X; NIP3L; NIX

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

1-219aa

Target Protein Sequence

MSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLKVFIPSLFLSHVLALGLGIYIGKRLSTPSASTY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

25.9kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Just like other recombinant proteins, the production of this recombinant Human BNIP3L protein began with appropriate cDNA and PCR methods, and then the BNIP3L expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into Yeast for the expression of the recombinant Human BNIP3L protein. N-terminal 6xHis tag was used in the process. And we finally get the protein of interest with purity of 90%+.

BNIP3L (also called BNIP3A, BNIP3H, NIX) is a gene encoding a protein called BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (also known as adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L) in human. The protein encoded by BNP3L gene is a mitochondrial protein from the outer membrane and belongs to the BH3-only protein from the BCL2 family. BNIP3L was initially recognized as a proapoptotic protein with milder efficacy in inducing apoptosis compared to other proteins in this family.

Target Background

Function

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

Gene References into Functions

The results demonstrate that Nix can serve as an alternative mediator of mitophagy to maintain mitochondrial turnover, identifying Nix as a promising target for neuroprotective treatment in PINK1/Parkin-related Parkinson's disease. PMID: 28281653
Here the authors present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. PMID: 28442745
p75(NTR) and NIX may play critical roles in intracerebral hemorrhage-induced neuronal apoptosis in vitro and in vivo. PMID: 27726026
Data suggest that targeting BNIP3L protein in wild-type p53 tumor suppressor colon cancer cells is an anticancer strategy activating iron depletion signaling and the mitophagy-related cell death pathway. PMID: 26517689
regulates mitophagy during hypoxia, whereas NIX is required for mitophagy during development of the erythroid lineage. PMID: 25753537
Nix protein positively regulates NF-kappaB activation in gliomas PMID: 22984526
mitochondrial ROS and NIX are essential factors for Mieap-induced accumulation of lysosome-like organelles within mitochondria PMID: 21264228
Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation PMID: 20010802
NIX binds to TSAP6 in tumor cells and has a role in apoptosis PMID: 12606722
The proapoptotic factor Nix is a highly regulated effector of growth during terminal erythroid maturation. PMID: 12663450
Bnip3L, capable of attenuating tumorigenicity, mediates p53-dependent apoptosis under hypoxia. PMID: 15607964
NIX is required for the selective elimination of mitochondria, however, because mitochondrial clearance, in the absence of NIX, is arrested at the stage of mitochondrial incorporation into autophagosomes and autophagosome maturation. PMID: 18048346
Homozygous deletions in a number of biologically important genes were found in prostate cancer cell lines, including PPP2R2A and BNIP3L identified in this study. PMID: 18670647

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Subcellular Location

Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane; Single-pass membrane protein. Note=Colocalizes with SPATA18 at the mitochondrion outer membrane.

Protein Families

NIP3 family

Database Links

HGNC: 1085

OMIM: 605368

KEGG: hsa:665

STRING: 9606.ENSP00000370003

UniGene: Hs.131226

Code	CSB-YP002767HU
Abbreviation	Recombinant Human BNIP3L protein
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP002767HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) BNIP3L. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP002767HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) BNIP3L.
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Recombinant Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L)

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